Hephaestin antibody (AA 348-1157)

Catalog No. ABIN7997231

The Rabbit Polyclonal anti-Hephaestin antibody (ABIN7997231) specifically detects Hephaestin in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Hephaestin antibody (AA 348-1157) (ABIN7997231)

Target: Hephaestin (HEPH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hephaestin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Hephaestin Antibody

Binding Specificity: AA 348-1157

Purpose: Anti-Hephaestin/HEPH Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Mouse Hephaestin,HEPHshares 85.6%,96.2% amino acid (aa) sequence identity with human,rat Hephaestin,HEPH,respectively.

Characteristics: Anti-Hephaestin/HEPH Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hephaestin/HEPH recombinant protein (Position: E348-Q1157). Mouse Hephaestin/HEPHshares 85.6% and 96.2% amino acid (aa) sequence identity with human and rat Hephaestin/HEPH, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Hephaestin

Target: Hephaestin (HEPH)

Alternative Name: HEPH

Background:

Background: Enables ferroxidase activity. Involved in intestinal iron absorption, multicellular organismal-level iron ion homeostasis, and positive regulation of iron export across plasma membrane. Acts upstream of or within erythrocyte differentiation. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in basolateral plasma membrane. Orthologous to human HEPH (hephaestin).

Gene Full Name: hephaestin

Molecular Weight: 130 kDa

Gene ID: 15203

UniProt: Q9Z0Z4

Pathways: Transition Metal Ion Homeostasis