ChT antibody (AA 446-580)

Catalog No. ABIN7997529

This Rabbit Polyclonal antibody specifically detects ChT in WB, FACS and ELISA. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for ChT antibody (AA 446-580) (ABIN7997529)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ChT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Grade: Carrier-free

Product Details anti-ChT Antibody

Binding Specificity: AA 446-580

Purpose: Anti-SLC5A7 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SLC5A7 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human ELISA, 0.1-0.5 μg/mL/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: SLC5A7

Background:

Background: The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene. This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.

Gene Full Name: solute carrier family 5 member 7

Molecular Weight: 80 kDa

Gene ID: 60482

UniProt: Q9GZV3