Integrin beta 4 antibody (AA 28-266) (APC)

Catalog No. ABIN8000601

The Rabbit Polyclonal anti-Integrin beta 4 antibody is suitable to detect Integrin beta 4 in samples from Human. It has been validated for FACS.

Quick Overview for Integrin beta 4 antibody (AA 28-266) (APC) (ABIN8000601)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 4 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-Integrin beta 4 Antibody (APC)

Binding Specificity: AA 28-266

Purpose: Anti-Integrin beta 4/ITGB4 Antibody APC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Integrin beta 4 shares 88%,86% amino acid (aa) sequences identity with mouse,rat Integrin beta 4,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: ITGB4

Background:

Background: ITGB4 (Integrin, beta-4), also known as CD104 (Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.

Gene Full Name: integrin subunit beta 4

Sequence Similarities: Belongs to the integrin beta chain family.

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex