ILK antibody (AA 1-203)

Catalog No. ABIN8000670

The Rabbit Polyclonal anti-ILK antibody is suitable to detect ILK in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for ILK antibody (AA 1-203) (ABIN8000670)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-ILK Antibody

Binding Specificity: AA 1-203

Purpose: Anti-Integrin linked ILK Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse,rat Integrin linked ILK.

Characteristics: Anti-Integrin linked ILK Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin linked ILK recombinant protein (Position: M1-H203). Human Integrin linked ILK shares 99.5% amino acid (aa) sequence identity with both mouse and rat Integrin linked ILK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK

Background:

Background: ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

Gene Full Name: integrin linked kinase

Molecular Weight: 55 kDa

Gene ID: 3611

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration