ILKAP antibody (AA 114-436)

Catalog No. ABIN8000714

This Rabbit Polyclonal antibody specifically detects ILKAP in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for ILKAP antibody (AA 114-436) (ABIN8000714)

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILKAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ILKAP Antibody

Binding Specificity: AA 114-436

Purpose: Anti-ILKAP Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PDK1 shares 94.7%,95.7% amino acid (aa) sequence identity with mouse,rat PDK1,respectively.

Characteristics: Anti-ILKAP Antibody. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDK1 recombinant protein (Position: Y114-A436). Human PDK1 shares 94.7% and 95.7% amino acid (aa) sequence identity with mouse and rat PDK1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ILKAP

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Alternative Name: ILKAP

Background:

Background: Integrin-linked kinase-associated serine/threonine phosphatase 2C is an enzyme that in humans is encoded by the ILKAP gene. The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Gene Full Name: ILK associated serine/threonine phosphatase

Molecular Weight: 43 kDa

Gene ID: 80895

UniProt: Q9H0C8