IRF8 antibody (C-Term)

Catalog No. ABIN8001330

The Rabbit Polyclonal anti-IRF8 antibody is suitable to detect IRF8 in samples from Human and Mouse. It has been validated for WB, IHC, FACS, ICC and IF.

Quick Overview for IRF8 antibody (C-Term) (ABIN8001330)

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-IRF8 Antibody

Binding Specificity: C-Term

Purpose: Anti-Interferon regulatory factor 8 IRF8 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by two amino acids.

Characteristics: Anti-Interferon regulatory factor 8 IRF8 Antibody. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IRF8, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IRF8

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Alternative Name: IRF8

Background:

Background: Interferon regulatory factor 8 (IRF8) also known as Interferon consensus sequence-binding protein (ICSBP), is a protein that in humans is encoded by the IRF8 gene. By genomic sequence analysis, the IRF8 gene is mapped to chromosome 16q24.1. Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 is a transcription factor that plays critical roles in the regulation of lineage commitment and in myeloid cell maturation including the decision for a common myeloid progenitor (CMP) to differentiate into a monocyte precursor cell.

Gene Full Name: interferon regulatory factor 8

Sequence Similarities: Belongs to the IRF family.

Molecular Weight: 48 kDa

Gene ID: 3394

UniProt: Q02556

Pathways: Cellular Response to Molecule of Bacterial Origin