IFT74 antibody (AA 1-375)

Catalog No. ABIN8003145

The Rabbit Polyclonal anti-IFT74 antibody is suitable to detect IFT74 in samples from Human, Rat, Mouse and Monkey. It has been validated for ELISA, WB, IHC and FACS.

Quick Overview for IFT74 antibody (AA 1-375) (ABIN8003145)

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT74 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-IFT74 Antibody

Binding Specificity: AA 1-375

Purpose: Anti-IFT74 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3.

Characteristics: Anti-IFT74 Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Alternative Name: IFT74

Background:

Background: Intraflagellar transport protein 74 homolog (IFT74), also known as coiled-coil domain-containing protein 2 (CCDC2) and capillary morphogenesis gene 1 protein (CMG1), is a protein that in humans is encoded by the IFT74 gene. This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome.

Gene Full Name: intraflagellar transport 74

Molecular Weight: 69 kDa

Gene ID: 80173

UniProt: Q96LB3

Pathways: Hedgehog Signaling, Chromatin Binding