ISL1 antibody (AA 118-161)

Catalog No. ABIN8003332

The Rabbit Polyclonal anti-ISL1 antibody (ABIN8003332) specifically detects ISL1 in WB, ELISA, IHC, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ISL1 antibody (AA 118-161) (ABIN8003332)

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-ISL1 Antibody

Binding Specificity: AA 118-161

Purpose: Anti-Islet 1/ISL1 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human ISL1 shares 100% amino acid (aa) sequence identity with both mouse,rat ISL1.

Characteristics: Anti-Islet 1/ISL1 Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Islet 1/ISL1 recombinant protein (Position: D118-E161). Human ISL1 shares 100% amino acid (aa) sequence identity with both mouse and rat ISL1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ISL1

Target: ISL1 (ISL LIM Homeobox 1 (ISL1))

Alternative Name: ISL1

Background:

Background: Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Gene Full Name: ISL LIM homeobox 1

Molecular Weight: 42 kDa

Gene ID: 3670

UniProt: P61371

Pathways: Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding