IDH3B antibody (AA 32-362)

Catalog No. ABIN8003464

The Rabbit Polyclonal anti-IDH3B antibody (ABIN8003464) specifically detects IDH3B in WB, ELISA, IHC, FACS and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IDH3B antibody (AA 32-362) (ABIN8003464)

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-IDH3B Antibody

Binding Specificity: AA 32-362

Purpose: Anti-IDH3B Antibody

Predicted Reactivity: Human IDH3B shares 97% amino acid (aa) sequence identity with rat IDH3B.

Characteristics: Anti-IDH3B Antibody. Tested in WB, IHC, Flow Cytometry, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IDH3B recombinant protein (Position: A32-D362). Human IDH3B shares 97% amino acid (aa) sequence identity with rat IDH3B.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human Immunoprecipitation, 0.5-2 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IDH3B

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Alternative Name: IDH3B

Background:

Background: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial is an enzyme that in humans is encoded by the IDH3B gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene Full Name: isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta

Molecular Weight: 42 kDa

Gene ID: 3420

UniProt: O43837