IDI1 antibody (AA 71-645)

Catalog No. ABIN8003508

The Rabbit Polyclonal anti-IDI1 antibody is suitable to detect IDI1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS and IHC.

Quick Overview for IDI1 antibody (AA 71-645) (ABIN8003508)

Target: IDI1 (Isopentenyl-Diphosphate delta Isomerase 1 (IDI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-IDI1 Antibody

Binding Specificity: AA 71-645

Purpose: Anti-IDI1 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human ATG7 shares 92.9% amino acid (aa) sequence identity with both mouse,rat ATG7.

Characteristics: Anti-IDI1 Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG7 recombinant protein (Position: D71-K645). Human ATG7 shares 92.9% amino acid (aa) sequence identity with both mouse and rat ATG7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IDI1

Target: IDI1 (Isopentenyl-Diphosphate delta Isomerase 1 (IDI1))

Alternative Name: IDI1

Background:

Background: IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity.

Gene Full Name: isopentenyl-diphosphate delta isomerase 1

Molecular Weight: 26-28 kDa

Gene ID: 3422

UniProt: Q13907