KIF3A antibody (AA 485-699) (Cy3)

Catalog No. ABIN8004489

The Rabbit Polyclonal anti-KIF3A antibody is suitable to detect KIF3A in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for KIF3A antibody (AA 485-699) (Cy3) (ABIN8004489)

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF3A antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-KIF3A Antibody (Cy3)

Binding Specificity: AA 485-699

Purpose: Anti-KIF3A Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human KIF3A shares 97.7% amino acid (aa) sequence identity with mouse KIF3A.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KIF3A recombinant protein (Position: D485-Q699). Human KIF3A shares 97.7% amino acid (aa) sequence identity with mouse KIF3A.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for KIF3A

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Alternative Name: KIF3A

Background:

Background: Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the plus ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

Gene Full Name: kinesin family member 3A

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Gene ID: 11127

UniProt: Q9Y496

Pathways: Hedgehog Signaling