KLF5 antibody (Middle Region) (HRP)

Catalog No. ABIN8004759

The Rabbit Polyclonal anti-KLF5 antibody is suitable to detect KLF5 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for KLF5 antibody (Middle Region) (HRP) (ABIN8004759)

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF5 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-KLF5 Antibody (HRP)

Binding Specificity: Middle Region

Purpose: Anti-Krueppel-like factor 5 KLF5 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human KLF5, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for KLF5

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Alternative Name: KLF5

Background:

Background: KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al. (2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al. (1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59 % and 64 % identical to those of Sp1 (189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene fused to a GC box-containing promoter. Sogawa et al. (1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

Gene Full Name: KLF transcription factor 5

Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.

Gene ID: 688

UniProt: Q13887