LDHD antibody (AA 56-498)

Catalog No. ABIN8004939

The Rabbit Polyclonal anti-LDHD antibody is suitable to detect LDHD in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for LDHD antibody (AA 56-498) (ABIN8004939)

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-LDHD Antibody

Binding Specificity: AA 56-498

Purpose: Anti-LDHD Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-LDHD Antibody. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LDHD recombinant protein (Position: R56-Q498).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LDHD

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Alternative Name: LDHD

Background:

Background: Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54 kDa, and can be detected as 45-54 kDa.

Gene Full Name: lactate dehydrogenase D

Molecular Weight: 45-50 kDa

Gene ID: 197257

UniProt: Q86WU2

Pathways: Ribonucleoside Biosynthetic Process