Lamin B1 antibody (AA 266-583)

Catalog No. ABIN8005016

This Rabbit Polyclonal antibody specifically detects Lamin B1 in WB, IHC, IP, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Lamin B1 antibody (AA 266-583) (ABIN8005016)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Lamin B1 Antibody

Binding Specificity: AA 266-583

Purpose: Anti-Lamin B1/LMNB1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Lamin B1 shares 95.9%,95% amino acid (aa) sequence identity with mouse,rat Lamin B1,respectively.

Characteristics: Anti-Lamin B1/LMNB1 Antibody. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL Immunoprecipitation, 0.5-2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: LMNB1

Background:

Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Gene Full Name: lamin B1

Sequence Similarities: Belongs to the intermediate filament family.

Molecular Weight: 67 kDa

Gene ID: 4001

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis