Laminin gamma 1 antibody

Catalog No. ABIN8005071

The Rabbit Polyclonal anti-Laminin gamma 1 antibody is suitable to detect Laminin gamma 1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for Laminin gamma 1 antibody (ABIN8005071)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Laminin gamma 1 Antibody

Purpose: Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody

Sequence: NKLNEIEGSL NKAKDEMKAS ,DLEERVRRQR NHLHLLETSI ,LQLDSHGALH HKLRQLEEES

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: Peptide mixture of laminin gamma1,2,3 (NKLNEIEGSLNKAKDEMKAS, DLEERVRRQRNHLHLLETSI, LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat Immunofluorescence, 2 μg/mL, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: Lamc1

Background:

Background: Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Gene Full Name: Laminin subunit gamma-1

Molecular Weight: 150, 220-250 kDa

Gene ID: 226519

UniProt: P02468