LCAT antibody (C-Term)

Catalog No. ABIN8005170

The Rabbit Polyclonal anti-LCAT antibody is suitable to detect LCAT in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for LCAT antibody (C-Term) (ABIN8005170)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCAT antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-LCAT Antibody

Binding Specificity: C-Term

Purpose: Anti-LCAT Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related human sequence by six amino acids,and from the related rat sequence by four amino acids.

Characteristics: Anti-LCAT Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse LCAT, different from the related human sequence by six amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: Lcat

Background:

Background: LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed 'reverse cholesterol transport.' The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).

Gene Full Name: lecithin cholesterol acyltransferase

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 50 kDa

Gene ID: 16816

UniProt: P16301

Pathways: Lipid Metabolism