LCAT antibody (C-Term) (HRP)

Catalog No. ABIN8005177

The HRP-conjugated Rabbit Polyclonal anti-LCAT antibody (ABIN8005177) specifically detects LCAT in WB, IHC and ELISA. The antibody is reactive with Human and Rat samples.

Quick Overview for LCAT antibody (C-Term) (HRP) (ABIN8005177)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCAT antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-LCAT Antibody (HRP)

Binding Specificity: C-Term

Purpose: Anti-LCAT Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by two amino acids,and from the mouse sequence by three amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT, different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT

Background:

Background: LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).

Gene Full Name: lecithin-cholesterol acyltransferase

Sequence Similarities: Belongs to the AB hydrolase superfamily. Lipase family.

Gene ID: 3931

UniProt: P04180

Pathways: Lipid Metabolism