ME1 antibody (AA 1-572)

Catalog No. ABIN8007603

The Mouse Monoclonal anti-ME1 antibody is suitable to detect ME1 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for ME1 antibody (AA 1-572) (ABIN8007603)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Clone: 5E5F7

Product Details anti-ME1 Antibody

Binding Specificity: AA 1-572

Purpose: Anti-ME1 Antibody (monoclonal, 5E5F7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ME1 Antibody.(monoclonal, 5E5F7). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ME1 recombinant protein (Position: M1-Q572).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1

Background:

Background: NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Gene Full Name: malic enzyme 1

Molecular Weight: 64 kDa

Gene ID: 4199

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha