MPI antibody (AA 2-99)

Catalog No. ABIN8007691

The Mouse Monoclonal anti-MPI antibody (Clone 5G5) (ABIN8007691) specifically detects MPI in WB, FACS, IHC, IF and ICC. The antibody is reactive with Human and Rat samples.

Quick Overview for MPI antibody (AA 2-99) (ABIN8007691)

Target: MPI (Mannose Phosphate Isomerase (MPI))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MPI antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 5G5

Product Details anti-MPI Antibody

Binding Specificity: AA 2-99

Purpose: Anti-MPI Antibody (monoclonal, 5G5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8%,86.7% amino acid (aa) sequence identity with mouse,rat MPI,respectively.

Characteristics: Anti-MPI.Antibody (monoclonal, 5G5)-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MPI

Target: MPI (Mannose Phosphate Isomerase (MPI))

Alternative Name: MPI

Background:

Background: Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.

Gene Full Name: mannose phosphate isomerase

Molecular Weight: 45 kDa

Gene ID: 4351

UniProt: P34949