MCAM antibody (AA 59-401) (HRP)

Catalog No. ABIN8008919

This Mouse Monoclonal antibody specifically detects MCAM in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MCAM antibody (AA 59-401) (HRP) (ABIN8008919)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to HRP

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: 4C12

Product Details anti-MCAM Antibody (HRP)

Binding Specificity: AA 59-401

Purpose: Anti-CD146/MCAM Antibody (monoclonal, 4C12) HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human CD146 shares 73% amino acid (aa) sequence identity with both mouse,rat CD146.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.

Isotype: IgG2a

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background:

Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113 kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

Gene Full Name: melanoma cell adhesion molecule

Gene ID: 4162

UniProt: P43121