MCAM antibody (AA 27-621) (Fluoro488)

Catalog No. ABIN8008937

This Rabbit Polyclonal antibody specifically detects MCAM in FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for MCAM antibody (AA 27-621) (Fluoro488) (ABIN8008937)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAM antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-MCAM Antibody (Fluoro488)

Binding Specificity: AA 27-621

Purpose: Anti-CD146/Mcam Antibody Fluoro488 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD146/Mcam recombinant protein (Position: E27-E621).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: Mcam

Background:

Background: In humans, the CD146 protein is encoded by the MCAM gene. It is mapped to 9, 9 A5.1. CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113 kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM functions as a receptor for laminin alpha 4, a matrix molecule that is broadly expressed within the vascular wall. Accordingly, MCAM is highly expressed by cells that are components of the blood vessel wall, including vascular endothelial cells, smooth muscle cells and pericytes. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.

Gene Full Name: melanoma cell adhesion molecule

Gene ID: 84004

UniProt: Q8R2Y2