MBD2 antibody (AA 159-411)

Catalog No. ABIN8009583

The Rabbit Polyclonal anti-MBD2 antibody is suitable to detect MBD2 in samples from Human. It has been validated for WB.

Quick Overview for MBD2 antibody (AA 159-411) (ABIN8009583)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD2 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-MBD2 Antibody

Binding Specificity: AA 159-411

Purpose: Anti-MBD2 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.

Characteristics: Anti-MBD2 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Alternative Name: MBD2

Background:

Background: Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Gene Full Name: methyl-CpG binding domain protein 2

Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

Molecular Weight: 47 kDa

Gene ID: 8932

UniProt: Q9UBB5

Pathways: Chromatin Binding