MFAP3L antibody (AA 29-409) (Fluoro488)

Catalog No. ABIN8009971

The Fluoro488-conjugated Rabbit Polyclonal anti-MFAP3L antibody (ABIN8009971) specifically detects MFAP3L in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MFAP3L antibody (AA 29-409) (Fluoro488) (ABIN8009971)

Target: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFAP3L antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-MFAP3L Antibody (Fluoro488)

Binding Specificity: AA 29-409

Purpose: Anti-MFAP3L Antibody Fluoro488 Conjugated

Predicted Reactivity: Human MFAP3L shares 90.6% amino acid (aa) sequence identity with both mouse,rat MFAP3L.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MFAP3L recombinant protein (Position: K29-V409). Human MFAP3L shares 90.6% amino acid (aa) sequence identity with both mouse and rat MFAP3L.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for MFAP3L

Target: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Alternative Name: MFAP3L

Background:

Background: Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non-fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly. In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions . Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other. Human MFAP3L shows 71 % amino acid (aa) sequence homology to MFAP3, but not other MFAPs. Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif. The ECD of human MFAP3L shares 89 % and 90 % aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin. MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression. MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation. Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression.

Gene Full Name: microfibril associated protein 3 like

Gene ID: 9848

UniProt: O75121