MRPS18B antibody (AA 23-211)

Catalog No. ABIN8010739

The Rabbit Polyclonal anti-MRPS18B antibody (ABIN8010739) specifically detects MRPS18B in WB, IHC, ELISA, FACS, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for MRPS18B antibody (AA 23-211) (ABIN8010739)

Target: MRPS18B (Mitochondrial Ribosomal Protein S18B (MRPS18B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS18B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-MRPS18B Antibody

Binding Specificity: AA 23-211

Purpose: Anti-MRPS18B Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MRPS18B Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MRPS18B recombinant protein (Position: H23-R211).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPS18B

Target: MRPS18B (Mitochondrial Ribosomal Protein S18B (MRPS18B))

Alternative Name: MRPS18B

Background:

Background: 28S ribosomal protein S18b, mitochondrial is a protein that in humans is encoded by the MRPS18B gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.

Gene Full Name: mitochondrial ribosomal protein S18B

Molecular Weight: 26 kDa

Gene ID: 28973

UniProt: Q9Y676