MAP4K4 antibody (Middle Region)

Catalog No. ABIN8011399

The Rabbit Polyclonal anti-MAP4K4 antibody (ABIN8011399) specifically detects MAP4K4 in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MAP4K4 antibody (Middle Region) (ABIN8011399)

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-MAP4K4 Antibody

Binding Specificity: Middle Region

Purpose: Anti-MAP4K4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MAP4K4 Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MAP4K4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAP4K4

Target: MAP4K4 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4))

Alternative Name: MAP4K4

Background:

Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Full Name: mitogen-activated protein kinase kinase kinase kinase 4

Molecular Weight: 160 kDa

Gene ID: 9448

UniProt: O95819