MAG antibody (AA 34-605) (HRP)

Catalog No. ABIN8012352

The Mouse Monoclonal anti-MAG antibody is suitable to detect MAG in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for MAG antibody (AA 34-605) (HRP) (ABIN8012352)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAG antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 2G11

Product Details anti-MAG Antibody (HRP)

Binding Specificity: AA 34-605

Purpose: Anti-MAG Antibody (monoclonal, 2G11) HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAG recombinant protein (Position: E34-R605).

Isotype: IgG2a

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG

Background:

Background: MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.

Gene Full Name: myelin associated glycoprotein

Gene ID: 4099

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway