MAG antibody (AA 34-605)

Catalog No. ABIN8012356

The Mouse Monoclonal anti-MAG antibody (Clone 2G11) (ABIN8012356) specifically detects MAG in WB, IHC, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MAG antibody (AA 34-605) (ABIN8012356)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Clone: 2G11

Product Details anti-MAG Antibody

Binding Specificity: AA 34-605

Purpose: Anti-MAG Antibody (monoclonal, 2G11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAG.Antibody (monoclonal, 2G11). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAG recombinant protein (Position: E34-R605).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse,Rat Immunofluorescence, 5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: MAG

Background:

Background: MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.

Gene Full Name: myelin associated glycoprotein

Molecular Weight: 100 kDa

Gene ID: 4099

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway