NANS antibody (AA 1-341)

Catalog No. ABIN8013017

This Rabbit Polyclonal antibody specifically detects NANS in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for NANS antibody (AA 1-341) (ABIN8013017)

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NANS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NANS Antibody

Binding Specificity: AA 1-341

Purpose: Anti-NANS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NANS shares 96.2% amino acid (aa) sequence identity with mouse NANS.

Characteristics: Anti-NANS Antibody. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NANS recombinant protein (Position: M1-E341). Human NANS shares 96.2% amino acid (aa) sequence identity with mouse NANS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NANS

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Alternative Name: NANS

Background:

Background: Sialic acid synthase is an enzyme that in humans is encoded by the NANS gene. This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively, however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Gene Full Name: N-acetylneuraminate synthase

Molecular Weight: 38 kDa

Gene ID: 54187

UniProt: Q9NR45