NSDHL antibody (AA 7-373) (FITC)

Catalog No. ABIN8013365

This Rabbit Polyclonal antibody specifically detects NSDHL in WB, IHC, ELISA, IF, IP and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NSDHL antibody (AA 7-373) (FITC) (ABIN8013365)

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSDHL antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-NSDHL Antibody (FITC)

Binding Specificity: AA 7-373

Purpose: Anti-NSDHL Antibody FITC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NSDHL shares 83.4%,87.3% amino acid (aa) sequence identity with mouse,rat NSDHL,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSDHL recombinant protein (Position: E7-K373). Human NSDHL shares 83.4% and 87.3% amino acid (aa) sequence identity with mouse and rat NSDHL, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for NSDHL

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Alternative Name: NSDHL

Background:

Background: The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.

Gene Full Name: NAD(P) dependent 3-beta-hydroxysteroid dehydrogenase NSDHL

Gene ID: 50814

UniProt: Q15738