NSDHL antibody (AA 7-373)

Catalog No. ABIN8013370

The Rabbit Polyclonal anti-NSDHL antibody (ABIN8013370) specifically detects NSDHL in WB, ELISA, IHC, IF, IP and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NSDHL antibody (AA 7-373) (ABIN8013370)

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSDHL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-NSDHL Antibody

Binding Specificity: AA 7-373

Purpose: Anti-NSDHL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NSDHL shares 83.4%,87.3% amino acid (aa) sequence identity with mouse,rat NSDHL,respectively.

Characteristics: Anti-NSDHL Antibody. Tested in WB, IHC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSDHL recombinant protein (Position: E7-K373). Human NSDHL shares 83.4% and 87.3% amino acid (aa) sequence identity with mouse and rat NSDHL, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NSDHL

Target: NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))

Alternative Name: NSDHL

Background:

Background: The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.

Gene Full Name: NAD(P) dependent 3-beta-hydroxysteroid dehydrogenase NSDHL

Molecular Weight: 38 kDa

Gene ID: 50814

UniProt: Q15738