NOXA1 antibody (Middle Region) (FITC)

Catalog No. ABIN8013816

The Rabbit Polyclonal anti-NOXA1 antibody is suitable to detect NOXA1 in samples from Human. It has been validated for FACS.

Quick Overview for NOXA1 antibody (Middle Region) (FITC) (ABIN8013816)

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-NOXA1 Antibody (FITC)

Binding Specificity: Middle Region

Purpose: Anti-NOXA1 Antibody FITC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for NOXA1

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Alternative Name: NOXA1

Background:

Background: NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Gene Full Name: NADPH oxidase activator 1

Sequence Similarities: Belongs to the NCF2/NOXA1 family.

Gene ID: 10811

UniProt: Q86UR1