2B4 antibody (AA 20-372) (HRP)

Catalog No. ABIN8013993

The Rabbit Polyclonal anti-2B4 antibody is suitable to detect 2B4 in samples from Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for 2B4 antibody (AA 20-372) (HRP) (ABIN8013993)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 2B4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-2B4 Antibody (HRP)

Binding Specificity: AA 20-372

Purpose: Anti-2B4/Cd244 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat 2B4/Cd244 recombinant protein (Position: Q20-Q372).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: Cd244

Background:

Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al. (1999) mapped the 2B4 gene to 1q22. Suzuki et al. (2008) identified a functional single-nucleotide polymorphism (SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al. (1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al. (1998) demonstrated that CD48 binds to 2B4. Watzl et al. (2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.

Gene Full Name: CD244 Molecule

Gene ID: 64025

UniProt: E9PT21