N4BP1 antibody (AA 1-885) (Fluoro647)

Catalog No. ABIN8014128

The Rabbit Polyclonal anti-N4BP1 antibody is suitable to detect N4BP1 in samples from Human. It has been validated for FACS.

Quick Overview for N4BP1 antibody (AA 1-885) (Fluoro647) (ABIN8014128)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-N4BP1 Antibody (Fluoro647)

Binding Specificity: AA 1-885

Purpose: Anti-N4BP1 Antibody Fluoro647 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human N4BP1 recombinant protein (Position: M1-K885).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1

Background:

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Gene Full Name: NEDD4 binding protein 1

Gene ID: 9683

UniProt: O75113