N4BP1 antibody (AA 1-885)

Catalog No. ABIN8014129

The Rabbit Polyclonal anti-N4BP1 antibody (ABIN8014129) specifically detects N4BP1 in WB, ELISA, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for N4BP1 antibody (AA 1-885) (ABIN8014129)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N4BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-N4BP1 Antibody

Binding Specificity: AA 1-885

Purpose: Anti-N4BP1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-N4BP1 Antibody. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human N4BP1 recombinant protein (Position: M1-K885).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Alternative Name: N4BP1

Background:

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Gene Full Name: NEDD4 binding protein 1

Molecular Weight: 110 kDa

Gene ID: 9683

UniProt: O75113