Nestin antibody (N-Term)

Catalog No. ABIN8014371

The Rabbit Polyclonal anti-Nestin antibody is suitable to detect Nestin in samples from Human, Mouse and Rat. It has been validated for IHC and WB.

Quick Overview for Nestin antibody (N-Term) (ABIN8014371)

Target: Nestin (NES)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Nestin Antibody

Binding Specificity: N-Term

Purpose: Anti-Nestin Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by four amino acids,and from the related rat sequence by five amino acids.

Characteristics: Anti-Nestin Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mLImmunohistochemistry (Frozen Section), 0.5-1 μg/mL Western blot, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES

Background:

Background: Nestin is a type VI intermediate filament  (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Gene Full Name: nestin

Molecular Weight: 230 kDa

Gene ID: 10763

UniProt: P48681