Netrin 1 antibody (N-Term) (Fluoro647)

Catalog No. ABIN8014381

The Fluoro647-conjugated Rabbit Polyclonal anti-Netrin 1 antibody (ABIN8014381) specifically detects Netrin 1 in FACS. The antibody is reactive with Mouse, Human and Rat samples.

Quick Overview for Netrin 1 antibody (N-Term) (Fluoro647) (ABIN8014381)

Target: Netrin 1 (NTN1)

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-Netrin 1 Antibody (Fluoro647)

Binding Specificity: N-Term

Purpose: Anti-Netrin 1/NTN1 Antibody Fluoro647 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: NTN1

Background:

Background: Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons ly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.

Gene Full Name: netrin 1

Sequence Similarities: Contains 3 laminin EGF-like domains.

Gene ID: 9423

UniProt: O95631

Pathways: Regulation of Cell Size