NAB2 antibody (AA 235-525)

Catalog No. ABIN8015361

The Rabbit Polyclonal anti-NAB2 antibody is suitable to detect NAB2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS, ICC and IF.

Quick Overview for NAB2 antibody (AA 235-525) (ABIN8015361)

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-NAB2 Antibody

Binding Specificity: AA 235-525

Purpose: Anti-NAB2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NAB2 shares 96.6% amino acid (aa) sequence identity with mouse NAB2.

Characteristics: Anti-NAB2 Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NAB2 recombinant protein (Position: D235-Q525). Human NAB2 shares 96.6% amino acid (aa) sequence identity with mouse NAB2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NAB2

Target: NAB2 (NGFI-A Binding Protein 2 (EGR1 Binding Protein 2) (NAB2))

Alternative Name: NAB2

Background:

Background: NGFI-A-binding protein 2 also known as EGR-1-binding protein 2 or melanoma-associated delayed early response protein (MADER) is a protein that in humans is encoded by the NAB2 gene. This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Gene Full Name: NGFI-A binding protein 2

Molecular Weight: 57 kDa

Gene ID: 4665

UniProt: Q15742