NSMCE4A antibody (AA 69-380)

Catalog No. ABIN8016076

The Rabbit Polyclonal anti-NSMCE4A antibody (ABIN8016076) specifically detects NSMCE4A in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NSMCE4A antibody (AA 69-380) (ABIN8016076)

Target: NSMCE4A (Non-SMC Element 4 Homolog A (NSMCE4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-NSMCE4A Antibody

Binding Specificity: AA 69-380

Purpose: Anti-NSMCE4A Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-NSMCE4A Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSMCE4A recombinant protein (Position: D69-Q380).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NSMCE4A

Target: NSMCE4A (Non-SMC Element 4 Homolog A (NSMCE4A))

Alternative Name: NSMCE4A

Background:

Background: Non-SMC element 4 homolog A is a protein that in humans is encoded by the NSMCE4A gene. NSMCE4A is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). It is involved in positive regulation of response to DNA damage stimulus.

Gene Full Name: NSE4A component of SMC5/6 complex

Molecular Weight: 50 kDa

Gene ID: 54780

UniProt: Q9NXX6

Pathways: Positive Regulation of Response to DNA Damage Stimulus