NFIB antibody (Middle Region) (Biotin)

Catalog No. ABIN8016386

This Mouse Monoclonal antibody specifically detects NFIB in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFIB antibody (Middle Region) (Biotin) (ABIN8016386)

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFIB antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4D6E4

Product Details anti-NFIB Antibody (Biotin)

Binding Specificity: Middle Region

Purpose: Anti-NFIB/NF1B2 Antibody (monoclonal, 4D6E4) Biotin Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NFIB/NF1B2, identical to the related mouse and rat sequences.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Alternative Name: NFIB

Background:

Background: Nuclear factor 1 B-type is a protein that in humans is encoded by the NFIB gene. The NFIB gene is a part of the NFI gene complex that includes three other genes (NFIA, NFIC and NFIX). The NFIB gene is a protein coding gene that also serves as a transcription factor. This gene is essential in embryonic development and it works together with its gene complex to initiate tissue differentiation in the fetus. Through knockout experiments, researchers found that mice without the NFIB gene have severely underdeveloped lungs. This mutation does not seem to cause spontaneous abortions because in utero the fetus does not use its lungs for respiration. However, this becomes lethal once the fetus is born and has to take its first breath. It is thought that NFIB plays a role in down regulating the transcription factors TGF-β1 and Shh in normal gestation because they remained high in knockout experiments. The absence of NFIB also leads to insufficient amounts of surfactant being produced which is one reason why the mice cannot breathe once it is born. The knockout experiments demonstrated that NFIB has a significant role in fore-brain development. NFIB is typically found in pontine nuclei of the CNS, the cerebral cortex and the white matter of the brain and without NFIB these areas are dramatically affected.

Gene Full Name: nuclear factor I B

Gene ID: 4781

UniProt: O00712