Osteocalcin antibody (AA 50-99)

Catalog No. ABIN8018523

The Rabbit Polyclonal anti-Osteocalcin antibody is suitable to detect Osteocalcin in samples from Rat. It has been validated for IHC and FACS.

Quick Overview for Osteocalcin antibody (AA 50-99) (ABIN8018523)

Target: Osteocalcin (BGLAP)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Osteocalcin Antibody

Binding Specificity: AA 50-99

Purpose: Anti-Osteocalcin/BGLAP Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9%,68.9% amino acid (aa) sequence identity with human,mouse Osteocalcin,respectively.

Characteristics: Anti-Osteocalcin/BGLAP Antibody. Tested in Flow Cytometry, IHC applications. This antibody reacts with Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Bglap

Background:

Background: Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time ing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation.

Gene Full Name: bone gamma-carboxyglutamate protein

Molecular Weight: 29 kDa, 60 kDa

Gene ID: 25295

UniProt: P04640