PARK7/DJ1 antibody (AA 2-189)

Catalog No. ABIN8019679

The Mouse Monoclonal anti-PARK7/DJ1 antibody (Clone 4B10) (ABIN8019679) specifically detects PARK7/DJ1 in WB, IHC, ICC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for PARK7/DJ1 antibody (AA 2-189) (ABIN8019679)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARK7/DJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: 4B10

Product Details anti-PARK7/DJ1 Antibody

Binding Specificity: AA 2-189

Purpose: Anti-PARK7/DJ1 Antibody (monoclonal, 4B10)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse,rat PARK7.

Characteristics: Anti-PARK7/DJ1 Antibody.(monoclonal, 4B10)-4. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Alternative Name: PARK7

Background:

Background: Parkinson disease (autosomal recessive, early onset) 7, also known as DJ1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. PARK7 is mapped to chromosome 1p36. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. This gene may also function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that PARK7 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.

Gene Full Name: Parkinsonism associated deglycase

Molecular Weight: 22 kDa

Gene ID: 11315

UniProt: Q99497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport