Pellino 1 antibody (N-Term)

Catalog No. ABIN8020005

The Rabbit Polyclonal anti-Pellino 1 antibody is suitable to detect Pellino 1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IP and FACS.

Quick Overview for Pellino 1 antibody (N-Term) (ABIN8020005)

Target: Pellino 1 (PELI1) (Pellino E3 Ubiquitin Protein Ligase 1 (PELI1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pellino 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Pellino 1 Antibody

Binding Specificity: N-Term

Purpose: Anti-PELI1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PELI1 Antibody. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PELI1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Pellino 1

Target: Pellino 1 (PELI1) (Pellino E3 Ubiquitin Protein Ligase 1 (PELI1))

Alternative Name: PELI1

Background:

Background: Enables ubiquitin-ubiquitin ligase activity. Involved in several processes, including negative regulation of TORC1 signaling, negative regulation of necroptotic process, and protein polyubiquitination. Is active in site of double-strand break. Implicated in colorectal cancer.

Gene Full Name: pellino E3 ubiquitin protein ligase 1

Molecular Weight: 40 kDa

Gene ID: 57162

UniProt: Q96FA3

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades