Peroxiredoxin 5 antibody (AA 66-198)

Catalog No. ABIN8020681

The Rabbit Polyclonal anti-Peroxiredoxin 5 antibody (ABIN8020681) specifically detects Peroxiredoxin 5 in WB, IHC, ICC, FACS and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Peroxiredoxin 5 antibody (AA 66-198) (ABIN8020681)

Target: Peroxiredoxin 5 (PRDX5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-Peroxiredoxin 5 Antibody

Binding Specificity: AA 66-198

Purpose: Anti-Peroxiredoxin 5/PRDX5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse,rat Peroxiredoxin 5.

Characteristics: Anti-Peroxiredoxin 5/PRDX5 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Peroxiredoxin 5

Target: Peroxiredoxin 5 (PRDX5)

Alternative Name: PRDX5

Background:

Background: PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Gene Full Name: peroxiredoxin 5

Sequence Similarities: Belongs to the peroxiredoxin 2 family.

Molecular Weight: 22 kDa

Gene ID: 25824

UniProt: P30044

Pathways: Cell RedoxHomeostasis