PHF8 antibody (AA 108-946)

Catalog No. ABIN8021053

The Rabbit Polyclonal anti-PHF8 antibody (ABIN8021053) specifically detects PHF8 in WB, ICC, FACS, IF and ELISA. The antibody is reactive with Human samples.

Quick Overview for PHF8 antibody (AA 108-946) (ABIN8021053)

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Grade: Carrier-free

Product Details anti-PHF8 Antibody

Binding Specificity: AA 108-946

Purpose: Anti-PHF8 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PHF8 shares 94.6% amino acid (aa) sequence identity with mouse PHF8.

Characteristics: Anti-PHF8 Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHF8 recombinant protein (Position: H108-D946). Human PHF8 shares 94.6% amino acid (aa) sequence identity with mouse PHF8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PHF8

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Alternative Name: PHF8

Background:

Background: The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: PHD finger protein 8

Molecular Weight: 140 kDa

Gene ID: 23133

UniProt: Q9UPP1