PEBP1 antibody (N-Term) (Fluoro550)

Catalog No. ABIN8021213

This Rabbit Polyclonal antibody specifically detects PEBP1 in FACS. It exhibits reactivity toward Mouse and Rat.

Quick Overview for PEBP1 antibody (N-Term) (Fluoro550) (ABIN8021213)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Product Details anti-PEBP1 Antibody (Fluoro550)

Binding Specificity: N-Term

Purpose: Anti-PBP/PEBP1 Antibody Fluoro550 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat sequence.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1

Background:

Background: PEBP1 (Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al (2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

Gene Full Name: phosphatidylethanolamine binding protein 1

Sequence Similarities: Belongs to the MCM family.

Gene ID: 23980

UniProt: P70296

Pathways: Feeding Behaviour