PI4KB antibody (AA 375-671) (PE)

Catalog No. ABIN8021263

The PE-conjugated Rabbit Polyclonal anti-PI4KB antibody (ABIN8021263) specifically detects PI4KB in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PI4KB antibody (AA 375-671) (PE) (ABIN8021263)

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI4KB antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-PI4KB Antibody (PE)

Binding Specificity: AA 375-671

Purpose: Anti-PI4KB Antibody PE Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PI4KB shares 100%,99.7% amino acid (aa) sequence identity with mouse,rat PI4KB,respectively

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PI4KB recombinant protein (Position: D375-D671). Human PI4KB shares 100% and 99.7% amino acid (aa) sequence identity with mouse and rat PI4KB, respectively

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PI4KB

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Alternative Name: PI4KB

Background:

Background: Phosphatidylinositol 4-kinase beta is an enzyme that in humans is encoded by the PI4KB gene. Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes. All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes. PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives.

Gene Full Name: phosphatidylinositol 4-kinase beta

Gene ID: 5298

UniProt: Q9UBF8

Pathways: Inositol Metabolic Process