PDE4D antibody (AA 466-709)

Catalog No. ABIN8021478

The Rabbit Polyclonal anti-PDE4D antibody is suitable to detect PDE4D in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS and ICC.

Quick Overview for PDE4D antibody (AA 466-709) (ABIN8021478)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-PDE4D Antibody

Binding Specificity: AA 466-709

Purpose: Anti-PDE4D Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDE4D Antibody. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PDE4D recombinant protein (Position: H466-Q709).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry, 0.5-1 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background:

Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4D is an enzyme that in humans is encoded by the PDE4D gene. This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

Gene Full Name: phosphodiesterase 4D

Molecular Weight: 91 kDa

Gene ID: 5144

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling