ATG4D antibody (AA 381-474) (Biotin)

Catalog No. ABIN752475

This anti-ATG4D antibody is a Rabbit Polyclonal antibody detecting ATG4D in WB, ELISA, IHC (p) and IHC (fro). Suitable for Mouse.

Quick Overview for ATG4D antibody (AA 381-474) (Biotin) (ABIN752475)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4D antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ATG4D Antibody (Biotin)

Binding Specificity: AA 381-474

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATG4D

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D

Background:

Synonyms: APG4D, AUTL4, APG4-D, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, ATG4D

Background: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Gene ID: 84971

UniProt: Q86TL0

Pathways: Autophagy