PIK3R4 antibody (AA 36-1339) (APC)

Catalog No. ABIN8021842

The APC-conjugated Rabbit Polyclonal anti-PIK3R4 antibody (ABIN8021842) specifically detects PIK3R4 in FACS. The antibody is reactive with Human samples.

Quick Overview for PIK3R4 antibody (AA 36-1339) (APC) (ABIN8021842)

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R4 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-PIK3R4 Antibody (APC)

Binding Specificity: AA 36-1339

Purpose: Anti-PIK3R4 Antibody APC Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PIK3R4 shares 95.7%,95.6% amino acid (aa) sequence identity with mouse,rat PIK3R4,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PIK3R4 recombinant protein (Position: R36-Q1339). Human PIK3R4 shares 95.7% and 95.6% amino acid (aa) sequence identity with mouse and rat PIK3R4, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PIK3R4

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Alternative Name: PIK3R4

Background:

Background: Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is ly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.

Gene Full Name: phosphoinositide-3-kinase regulatory subunit 4

Gene ID: 30849

UniProt: Q99570