Phospholamban antibody (N-Term)

Catalog No. ABIN8021977

The Rabbit Polyclonal anti-Phospholamban antibody (ABIN8021977) specifically detects Phospholamban in WB. The antibody is reactive with Rat and Mouse samples.

Quick Overview for Phospholamban antibody (N-Term) (ABIN8021977)

Target: Phospholamban (PLN)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Phospholamban Antibody

Binding Specificity: N-Term

Purpose: Anti-Phospholamban/PLN Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-Phospholamban/PLN Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PLN, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLN

Background:

Background: Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca (2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. McTiernan et al. (1999) observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.

Gene Full Name: phospholamban

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 24 kDa, 12 kDa

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity